Submitted
Last Updated August 9, 2022
Novel Measurement for Performance Improvement Challenge
Validation project
Team Leader
Seon-Ju Yeo
Solution Overview & Team Lead Details
Our Organization
Seoul National University
What is the name of your solution?
Validation project
Provide a one-line summary of your solution.
Efficient Plasmodium vivax-specific diagnostic biomaker using immuno-bioinformatic tools to develop on-site monitoring system
What specific problem are you solving?
There is a scarce of the highly sensitive rapid diagnostic system to detect Plasmodium species in field because there is a methodological limitation to identify P.vivax-specific biomarker caused from difficulty to culture P. vivax in vitro.
In this study, bioinformatic tools will be used for prediction of P.vivax-specific antigens based on information of antigenic determinants (B cell epitopes) by virtual bioinformatics tools.
What is your solution?
We propose the advanced method to develop a highly specific Plasmodum species -specific diagnostic system applicabla in the field by detecting P. vivax parasite itself as well as detecting P. vivax-specific IgM and IgG using efficiently cognitive P.vivax antigens which may possess potential epitopes.
Who does your solution serve, and in what ways will the solution impact their lives?
In everywhere where P.vivax is prevalent, highly sensitive and specific advanced diagnostic system will be helpful to manage patients and reduce patient loss.
How are you and your team well-positioned to deliver this solution?
My team has been investigating the understanding immuno-bioinformatic tools to define the parameters useful for predicting diagnostic antigen for dengue, zika, and malaria.
I understood that immuno bioinformatic tools were useful for effective antigen for dengue under prediction of tools. It made me confident and excited at applying this knowledge to P.vivax detection.
Which dimension of the Challenge does your solution most closely address?
- Employ unconventional or proxy data sources to inform primary health care performance improvement
- Provide improved measurement methods that are low cost, fit-for-purpose, shareable across information systems, and streamlined for data collectors
Where our solution team is headquartered or located:
Seoul, South Korea
Our solution's stage of development:
- Prototype
How many people does your solution currently serve?
2
Why are you applying to Solve?
I would like to support the global patients samples to test this techinical knowledge to predict the efficient antigen using immunobioinformatic tools.
Who is the Team Lead for your solution?
Professor
Page 3: More About Your Solution
What makes your solution innovative?
- Immuno-bioinformatic tools will be used for screening the highly antigenic and abundant of MHC binding peptide candidate antigen of P. vivax: IEDB.org for B-cell epitopes (tools of linear epitope analysis: ABCprep, BCPreds, BepiPred-2.0 and those of conformational epitope analysis: Ellipro, DiscoTope, BEpro ) and T-cell epitopes (T cell epitope prediction for MHC I-binding peptides and MHC II-binding peptides: EIDB Analysis resource: http://tools.iedb.org/main/tcell/.
- Highly potential antigens will be validated with patient specimens and the final antigens can be applied to portable rapid fluorescent strip kit which takes only 20 minutes to conduct assay. It could contribute to validation of various bioinformatic tools and in the future, many infectious diseases can be easily prevented and controlled by the advanced bioinformatic tools through this study.
- Rapid fluorescent strip kit will be optimized to be specific P.vivax parasite by detecting antigen and IgM/IgG.
What are your impact goals for the next year and the next five years, and how will you achieve them?
goals for the next year : I will test my predicted potential diagnosti antigen with global and Korea patient samples.
goals for the next five year :I will test my predicted potential diagnosti antigen-employing rapid fluorescent diagnostic system with global and Korea patient samples.
How are you measuring your progress toward your impact goals?
- Performance of rapid fluorescent diagnostic strip will be determined by virtual malaria patient specimens and the sensitivity of rapid strip kit will be compared to molecular diagnostic method such as RT-PCR.
What is your theory of change?
As P.vivax is infecting reticulocyte not RBC, the trace of parasite in blood is pretty low so definitely, it could contribute to confirmation in drug efficacy or vaccine developement by measuring the rest of parasite in blood.
Describe the core technology that powers your solution.
- Immuno-bioinformatic tools will be used for screening the highly antigenic and abundant of MHC binding peptide candidate antigen of P. vivax: IEDB.org for B-cell epitopes (tools of linear epitope analysis: ABCprep, BCPreds, BepiPred-2.0 and those of conformational epitope analysis: Ellipro, DiscoTope, BEpro ) and T-cell epitopes (T cell epitope prediction for MHC I-binding peptides and MHC II-binding peptides: EIDB Analysis resource: http://tools.iedb.org/main/tcell/.
- In our study, we confirmed that these bioinformatic tools were relevant to definining better diagnostic antigen in Dengue work (https://www.ncbi.nlm.nih.gov/p...).
Which of the following categories best describes your solution?
- A new application of an existing technology
Please select the technologies currently used in your solution:
- Biotechnology / Bioengineering
If your solution has a website or an app, provide the links here:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6679088/pdf/ijms-20-03464.pdf
Which of the UN Sustainable Development Goals does your solution address?
- 17. Partnerships for the Goals
Who collects the primary health care data for your solution?
In Korea, KCDC will provide patient samples.
Page 4: Your Team
What type of organization is your solution team?
- Nonprofit
How many people work on your solution team?
2 graduate students and 1 postdoc
How long have you been working on your solution?
9 years
What is your approach to incorporating diversity, equity, and inclusivity into your work?
Diversity: This study is for improving health care monitoring system for all global patients including Africa, America, and south East asia.
Equity: This study will have an advantage to manage malaria patient in all developing countries in low-cost.
Inclusion:If we succeed in developing the more efficient prediction of diagnostic antigen, we can contribute to preparation to all world-threatening highly pathogen in advance for all countries even in neglected area.
Page 5: Your Business Model & Funding
What is your business model?
Rapid diagnostic strip kit will be generated as business product. After we validate it by local clinical samples, we will expand the clinical study in global partnership.
Do you primarily provide products or services directly to individuals, to other organizations, or to the government?
- Organizations (B2B)
What is your plan for becoming financially sustainable?
Once we receive the reliable and high performance in different countries, patent will be pre-registered and then we will transfer our technology to company where is interested in our idea.
Share some examples of how your plan to achieve financial sustainability has been successful so far.
I received several grant continously to improve my experience and knowledge for developing rapid fluorescent diagnostic system targeting different infectious diseases (Zika, Dengue, Malaria, Avian infleunza virus).
My publications will show the evidence of my research capability to conduct this proposal.
- Yeo SJ, Huong DT, Han JH, Kim JY, Lee WJ, Shin HJ, Han ET, Park H. Performance of coumarin-derived dendrimer-based fluorescence-linked immunosorbent assay (FLISA) to detect malaria antigen. Malar J. 2014 Jul 10;13:266.
- Cowling RT, Yeo SJ, Kim IJ, Park JI, Gu Y, Dalton ND, Peterson KL, Greenberg BH. Discoidin domain receptor 2 germline gene deletion leads to altered heart structure and function in the mouse. Am J Physiol Heart Circ Physiol. 2014 Sep 1;307(5):H773-81.
- Seon-Ju Yeo, Dinh Thi Huong, Nguyen Ngoc Hong, Chun-Ying Li, Kyunghan Choi, Kyoungsik Yu, Du-Young Choi, Chom-Kyu Chong, Hak Soo Choi, Shyam Kumar Mallik, Hak Sung Kim, Haan Woo Sung, Hyun Park. Rapid and Quantitative Detection of Zoonotic Influenza A Virus Infection Utilizing Coumarin-derived dendrimer-based Fluorescent Immunochromatographic Strip Test (FICT). Theranostics 2014; 4(12):1239-1249
- Seon-Ju Yeo, Dong-Xu Liu, Hyun Park. Potential Interaction of Plasmodium falciparum Hsp60 and Calpain. Korean J Parasitol 2015 53(6): 665-673
- Seon-Ju Yeo, Hak Sung Kim, Suk Kim, Kee-In Lee, Hyun Park. Anticoccidial Effect of 3-(4-Methoxybenzylaminomethylene)-1,3-dihydroindole-2-one(5-108). The pharmaceutical society of Korea 2015 59 (60) 266-269
- Seon-Ju Yeo*1, Kyunghan Choi*2, Bui Thi Cuc, Nguyen Ngoc Hong, Duong Tuan Bao, Nguyen Minh Ngoc, Mai Quynh Le, Nguyen Le Khanh Hang, Nguyen Co Thach, Shyam Kumar Mallik, Hak Sung Kim, Chom-Kyu Chong, Hak Soo Choi, Haan Woo Sung, Kyoungsik Yu, Hyun Park. Smartphone-Based Fluorescent Diagnostic System for Highly Pathogenic H5N1 Viruses. Theranostics. 2016 Jan 1;6(2):231-42.
- Seon-Ju Yeo 1, Bui Thi Cuc 1, Haan Woo Sung 2, Hyun Park 3. Evaluation of a smartphone-based rapid fluorescent diagnostic system for H9N2 virus in specific-pathogen-free chickens. Arch Virol. 2016 Aug;161(8):2249-56.
- Seon-Ju Yeo1, ChunMei Jin2, SungYeon Kim3, Hyun Park1,*In Vitro and in Vivo Effects of Nitrofurantoin on Experimental Toxoplasmosis. Korean J Parasitol Vol. 54, No. 2: 155-161, April 2016
- Seon-Ju Yeo1‡, Dong-Xu Liu1‡, Hak Sung Kim2,Hyun Park1. Anti-malarial effect of novel chloroquine derivatives as agents for the treatment of malaria. Malaria Journal, 2017 16:80
- Duong Tuan Bao‡, Do Thi Hoang Kim‡, Hyun Park‡, Bui Thi Cuc, Nguyen Minh Ngoc, Nguyen Thi Phuong Linh, Nguyen Chien Huu, Trinh Thi Thuy Tien, Nguyen Thi Viet Anh, Tung Dao Duy, Chom-Kyu Chong, Seung-Taek Yu, Do-Young Choi, Seon-Ju Yeo. Rapid Detection of Avian Influenza Virus by Fluorescent Diagnostic Assay using an Epitope-Derived Peptide. Theranostics, 2017; 7(7):1835-1846
- Yeo SJ, Cuc BT, Kim SA, Kim DTH, Bao DT, Tien TTT, Anh NTV, Choi DY, Chong CK, Kim HS, Park H. Rapid detection of avian influenza A virus by immunochromatographic test using a novel fluorescent dye. Biosens Bioelectron. 2017 Aug 15;94:677-685
- Seon-Ju Yeo1‡, Duong Tuan Bao1‡, Ga-Eun Seo2, Bui Thi Cuc1, Do Thi Hoang Kim1, Nguyen Thi Viet Anh1, Trinh Thi Thuy Tien1, Nguyen Thi Phuong Linh1, Hae-Jin Sohn2, Chom-Kyu Chong3, Ho-Joon Shin2§, Hyun Park1§. Improvement of a rapid diagnostic application of monoclonal antibodies against avian influenza H7 subtype virus using Europium nanoparticles. Scientific report. 2017 7: 7933
- Nguyen Thi Phuong Linh1,†, Hyun Park1,† , Jinyoung Lee1,†, Dong-Xu Liu1, Ga-Eun Seo2, Hae-Jin Sohn2, Jin-Hee Han3, Eun-Taek Han3, Ho-Joon Shin2,*, Seon-Ju Yeo1,*Development of Monoclonal Antibodies for Diagnosis of Plasmodium vivax, Korean J Parasitol Vol. 55, No. 6: 623-630, December 2017
- Kim DTH, Bao DT, Park H, Ngoc NM, Yeo SJ. Development of a novel peptide aptamer-based immunoassay to detect Zika virus in serum and urine. Theranostics. 2018 Jun 7;8(13):3629-3642.
- Yu ST, Thi Bui C, Kim DTH, V T Nguyen A, Thi Trinh TT, Yeo SJ. Clinical evaluation of rapid fluorescent diagnostic immunochromatographic test for influenza A virus (H1N1). Scientific report. 2018 Sep 7;8(1):13468.
- Thuy Tien TT, Park H, Tuong HT, Yu ST, Choi DY, Yeo SJ. Development of a Rapid Fluorescent Immunochromatographic Test to Detect Respiratory Syncytial Virus. IntJ Mol Sci. 2018 Oct 2;19(10).
- Seon-Ju Yeo‡1, Homan Kang‡2, Tung Duy Dao ‡1, Bui Thi Cuc1, Anh Thi Viet Nguyen 1, Trinh Thi Thuy Tien1, Nguyen Le Khanh Hang3, Hoang Vu Mai Phuong3, Le Thi Thanh3, Le Quynh Mai3, Yoonhyuk Rah4, Kyoungsik Yu4, Ho-Joon Shin5, Chom-Kyu Chong6, Hak Soo Choi2, Hyun Park1. Development of a smartphone-based rapid dual fluorescent diagnostic system for the simultaneous detection of influenza A and H5 subtype in avian influenza A-infected patients. Theranostics. 2018; 8(22): 6132-6148.
- Dong-Xu Liu1, Trinh Thi Thuy Tien1, Duong Tuan Bao1, Nguyen Thi Phuong Linh, Hyun Park, Seon-Ju Yeo. A Novel Peptide Aptamer to Detect Plasmodium falciparum Lactate Dehydrogenase. Journal of Biomedical Nanotechnology 2019 ;15; 204-211.
- Anh Thi Viet Nguyena, Tien Thi Thuy Trinha, Vui Thi Hoanga, Tung Duy Dao, Hien Thi Tuong, Hak Sung Kim, Hyun Park, Seon-Ju Yeo. Peptide aptamer of complementarity-determining region to detect avian influenza virus. Journal of Biomedical Nanotechnology 2019 ;15 ; 1–16
- Nguyen NM, Duong BT, Azam M, Phuong TT, Park H, Thuy PTB, Yeo SJ. Diagnostic Performance of Dengue Virus Envelope Domain III in Acute Dengue Infection. Int J Mol Sci. 2019 Jul 15;20(14). pii: E3464.
- Nguyen HC, Park H, Shin HJ, Nguyen NM, Nguyen ATV, Trinh TT, Duong THY, Tuong HT, Hoang VT, Seo GE, Sohn HJ, Yeo SJ. Fluorescent Immunosorbent Assay for Chikungunya Virus Detection. Intervirology. 2019;62(3-4):145-155.
- Yeo SJ, Than DD, Park HS, Sung HW, Park H. Molecular Characterization of a Novel Avian Influenza A (H2N9) Strain Isolated from Wild Duck in Korea in 2018. Viruses. 2019 Nov 10;11(11).
- Hien Thi Tuong 1, Ngoc Minh Nguyen 1, Haan Woo Sung 2, Hyun Park 1,* and Seon-Ju Yeo1. Genetic Characterization of Avian Influenza A (H11N9) Virus Isolated from Mandarin Ducks in South Korea in 2018. Viruses 2020, Feb, 12, 203.
- Anh Thi Viet Nguyen*1, Tung Duy Dao*1,Thuy-Tien Thi Trinh*1, Du-Young Choi2, Seung-Taek Yu2, Hyun Park1, Seon-Ju Yeo§1. Sensitive Detection of Influenza A Virus Based on a CdSe/CdS/ZnS Quantum Dot-linked Rapid Fluorescent Immunochromatographic Test. Biosens Bioelectron. 2020 May 1;155:112090.
- Ngoc Minh Nguyen 1, Haan Woo Sung 2, Ki-Jung Yun 3, Hyun Park 1,* and Seon-Ju Yeo 1,*. Genetic Characterization of a Novel North American-Origin Avian Influenza A (H6N5) Virus Isolated from Bean Goose of South Korea in 2018.Viruses 2020, Jul 12(7), 774
- Thuy-Tien Thi Trinh 1, Bao Tuan Duong 1, Anh Thi Viet Nguyen 1, Hien Thi Tuong 1, Vui Thi Hoang 1, Duong Duc Than 1, Sun-Jeong Nam 2, Haan Woo Sung 3, Ki-Jung Yun 4, Seon-Ju Yeo 5*, Hyun Park 1*. Emergence of Novel Reassortant H1N1 Avian Influenza Viruses in Korean Wild Ducks in 2018 and 2019. Viruses. 2020 Dec 26;13(1):30.
- Trinh T-TT, Tiwari I, Durairaj K, Duong BT, Nguyen ATV, Tuong HT, Hoang VT, Than DD, Nam S, Yeo S-J, Park H. Genetic Characterization and Pathogenesis of Avian Influenza Virus H7N3 Isolated from Spot-Billed Ducks in South Korea, Early 2019. Viruses. 2021; 13(5):856
- Tuong HT, Jeong JH, Choi YK, Park H, Baek YH, Yeo SJ. Development of a Rapid Fluorescent Diagnostic System to Detect Subtype H9 Influenza A Virus in Chicken Feces. Int J Mol Sci. 2021 Aug 17;22(16):8823.
- Blagodatski A, Trutneva K, Glazova O, Mityaeva O, Shevkova L, Kegeles E, Onyanov N, Fede K, Maznina A, Khavina E, Yeo SJ, Park H, Volchkov P. Avian Influenza in Wild Birds and Poultry: Dissemination Pathways, Monitoring Methods, and Virus Ecology. Pathogens. 2021 May 20;10(5):630.
- Nguyen, A.T.V.; Hoang, V.T.; Sung, H.W.; Yeo, S.-J.; Park, H. Genetic Characterization and Pathogenesis of Three Novel Reassortant H5N2 Viruses in South Korea, 2018. Viruses 2021, 13, 2192
- Duong, B.T.; Bal, J.; Sung, H.W.; Yeo, S.-J.; Park, H. Molecular Analysis of the Avian H7 Influenza Viruses Circulating in South Korea during 2018–2019: Evolutionary Significance and Associated Zoonotic Threats. Viruses 2021, 13, 2260.
- Nguyen, A.T.V.; Hoang, V.T.; Sung, H.W.; Yeo, S.-J.; Park, H. Genetic Characterization and Pathogenesis of Three Novel Reassortant H5N2 Viruses in South Korea, 2018. Viruses 2021, 13, 2192.
- Duong BT, Bal J, Sung HW, Yeo SJ, Park H. Molecular Analysis of the Avian H7 Influenza Viruses Circulating in South Korea during 2018-2019: Evolutionary Significance and Associated Zoonotic Threats. Viruses. 2021 Nov 11;13(11):2260.
- Anh Thi Viet Nguyen, Bao Tuan Duong, Hyun Park, Seon-Ju Yeo. Development of a peptide aptamer pair-linked rapid fluorescent diagnostic system for Zika virus detection, Biosensors and Bioelectronics. 2022 Feb 197;1;113768
- Bao Tuan Duong, Duc Duong Than, Ulaankhuu Ankhanbaatar, Delgerzul Gombo-Ochir, Gansukh Shura, Amartuvshin Tsolmon, Ganzorig Basan, Seon Ju Yeo, Hyun Park. Assessing Potential Pathogenicity of Novel Highly Pathogenic Avian Influenza (H5N6) Viruses Isolated from Mongolian Wild Duck Feces Using a Mouse Model., Emerging Microbes & Infections, 2022 April, 22:1-29 25 May 2022
- Durairaj Kaliannan, Thuy-Tien Thi Trinh, Suyeon Yun, Seon-Ju Yeo *,Haan Woo Sung *, Hyun Park *. Molecular characterization and pathogenesis of H6N6 low pathogenic avian influenza viruses isolated from mallard ducks (Anas platyrhynchos) in South Korea. Viruses 2022, 14, 1001 8 May 2022
- Bao Tuan Duong1+, Duc Duong Than1+, Baeea Gum Ju1, Trinh Thi Thuy Tien2, Chris Ka Pun Mok4,5, Ju Hwan Jeong3 Min-Suk Song3, Yun Hee Baek3, Hyun Park1* and Seon-Ju Yeo. Development of a Rapid Fluorescent Diagnostic System for Early Detection of the Highly Pathogenic Avian Influenza H5 2.3.4.4 clade viruses in Chicken Stool. Int J Mol Sci. 2022 Jun 4;23(11):6301.
- Durairaj, K.; Than, D.D.; Nguyen, A.T.V.; Kim, H.S.; Yeo, S.-J.; Park, H. Cysteamine-Gold Coated Carboxylated Fluorescent Nanoparticle Mediated Point-of-Care Dual-Modality Detection of the H5N1 Pathogenic Virus. Int. J. Mol. Sci. 2022 July 19, 23, 7957.
Solution Team:
Seon-Ju Yeo